Description：Rabbit polyclonal antibody to PatchedImmunogen：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Patched. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol：PTCH1Alternative Names：PTCH; Protein patched homolog 1; PTC; PTC1 Entrez Gene (Human)： 5727; Entrez Gene (Mouse)： 19206; SwissProt (Human)： Q13635; SwissProt (Mouse)： Q61115; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Patched expression in HeLa (A), Ramos (B), MCF7 (C), NIH3T3 (D), PC12 (E) whole cell lysates. (Predicted band size: 160 kD; Observed band size: 160 kD)
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  Immunohistochemical analysis of Patched staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.