Description:Rabbit polyclonal antibody to PRKAR1BImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PRKAR1B. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:PRKAR1BAlternative Names:cAMP-dependent protein kinase type I-beta regulatory subunit
Entrez Gene (Human):
5575;
Entrez Gene (Mouse):
19085;
SwissProt (Human):
P31321;
SwissProt (Mouse):
P12849;
SwissProt (Rat):
P81377;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of PRKAR1B expression in HEK293T (A), U2OS (B), MCF7 (C), mouse liver (D) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 55 kD)
-
Immunohistochemical analysis of PRKAR1B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
-
Western blot analysis of PRKAR1B expression in A2780 (A), MG63 (B), Jurkat (C), mouse brain (D), mouse liver (E), rat brain (F), rat liver (G) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 55 kD)