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CPA1907
  • Western blot analysis of DNA Polymerase beta expression in HeLa (A), NIH3T3 (B), rat brain (C) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 38 kD)
  • Immunohistochemical analysis of DNA Polymerase beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-DNA Polymerase beta Antibody
Cat No:CPA1907
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to DNA Polymerase beta
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human DNA Polymerase beta. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol:POLB
Alternative Names:DNA polymerase beta
Entrez Gene (Human): 5423;
Entrez Gene (Mouse): 18970;
Entrez Gene (Rat): 29240;
SwissProt (Human): P06746;
SwissProt (Mouse): Q8K409;
SwissProt (Rat): P06766;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of DNA Polymerase beta expression in HeLa (A), NIH3T3 (B), rat brain (C) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 38 kD)
  • Immunohistochemical analysis of DNA Polymerase beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gastrointestinal hyperplasia with altered expression of DNA polymerase beta
Journal PLoS One
IF 3.7
Application IHC-P
Reactivity Human
PMID 19654874
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