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CPA1850
  • Western blot analysis of PAK3 (Phospho-S154) expression in BV2 (A), SKOVCAR3 (B), U87MG (C) whole cell lysates. (Predicted band size: 62 kD; Observed band size: 65 kD)
  • Immunohistochemical analysis of PAK3 (Phospho-S154) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-PAK3 (Phospho-S154) Antibody
Cat No:CPA1850
Source:Rabbit
Reactivity:H, M, R, Mk
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
420
100 μl
260
30 μl
130
50 μl
170
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Description:Rabbit polyclonal antibody to PAK3 (Phospho-S154)
Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S154 of human PAK3 protein. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)
Gene Symbol:PAK3
Alternative Names:OPHN3; Serine/threonine-protein kinase PAK 3; Beta-PAK; Oligophrenin-3; p21-activated kinase 3; PAK-3
Entrez Gene (Human): 5063;
Entrez Gene (Mouse): 18481;
Entrez Gene (Rat): 29433;
SwissProt (Human): O75914;
SwissProt (Mouse): Q61036;
SwissProt (Rat): Q62829;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of PAK3 (Phospho-S154) expression in BV2 (A), SKOVCAR3 (B), U87MG (C) whole cell lysates. (Predicted band size: 62 kD; Observed band size: 65 kD)
  • Immunohistochemical analysis of PAK3 (Phospho-S154) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The 5-phosphatase OCRL mediates retrograde transport of the mannose 6-phosphate receptor by regulating a Rac1-cofilin signalling module
Journal Hum Mol Genet
IF 3.5
Application WB
Reactivity Human
PMID 22907655
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