Description：Rabbit polyclonal antibody to PAHImmunogen：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PAH. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol：PAHAlternative Names：Phenylalanine-4-hydroxylase; PAH; Phe-4-monooxygenase Entrez Gene (Human)： 5053; Entrez Gene (Mouse)： 18478; SwissProt (Human)： P00439; SwissProt (Mouse)： P16331; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PAH expression in U2OS (A), DLD (B), mouse kidney (C), mouse liver (D) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 55 kD)
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  Immunohistochemical analysis of PAH staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.