Description：Rabbit polyclonal antibody to SIX6Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SIX6. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol：SIX6Alternative Names：OPTX2; SIX9; Homeobox protein SIX6; Homeodomain protein OPTX2; Optic homeobox 2; Sine oculis homeobox homolog 6 Entrez Gene (Human)： 4990; Entrez Gene (Mouse)： 20476; SwissProt (Human)： O95475; SwissProt (Mouse)： Q9QZ28; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SIX6 expression in HEK293T (A), HCT116 (B), mouse muscle (C), rat muscle (D) whole cell lysates. (Predicted band size: 35; 27 kD; Observed band size: 27; 43 kD)
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  Immunofluorescent analysis of SIX6 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.