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CPA1800
  • Western blot analysis of NF-kappaB p105/p50 (Phospho-S337) expression in Hela-TNFa (A) whole cell lysates. (Predicted band size: 105 kD; Observed band size: 105 kD)
  • Immunohistochemical analysis of NF-kappaB p105/p50 (Phospho-S337) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-NF-kappaB p105/p50 (Phospho-S337) Antibody
Cat No:CPA1800
Source:Rabbit
Reactivity:H, M, R, C, D, P
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
420
100 μl
260
30 μl
130
50 μl
170
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Description:Rabbit polyclonal antibody to NF-kappaB p105/p50 (Phospho-S337)
Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S337 of human NF-kappaB p105/p50 protein. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)
Gene Symbol:NFKB1
Alternative Names:Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Entrez Gene (Human): 4790;
Entrez Gene (Mouse): 18033;
SwissProt (Human): P19838;
SwissProt (Mouse): P25799;
SwissProt (Rat): Q63369;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of NF-kappaB p105/p50 (Phospho-S337) expression in Hela-TNFa (A) whole cell lysates. (Predicted band size: 105 kD; Observed band size: 105 kD)
  • Immunohistochemical analysis of NF-kappaB p105/p50 (Phospho-S337) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Therapeutic potential of andrographolide for treating endometriosis
Journal Hum Reprod
IF 6.1
Application IHC-P
Reactivity Rat
PMID 22402211
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