Description：Rabbit polyclonal antibody to NEDD8Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NEDD8. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol：NEDD8Alternative Names：NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8 Entrez Gene (Human)： 4738; Entrez Gene (Mouse)： 18002; Entrez Gene (Rat)： 25490; SwissProt (Human)： Q15843; SwissProt (Mouse)： P29595; SwissProt (Rat)： Q71UE8; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NEDD8 expression in mouse kidney (A), mouse testis (B), rat kidney (C) whole cell lysates. (Predicted band size: 9 kD; Observed band size: 18 kD)
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  Immunohistochemical analysis of NEDD8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.