Description:Rabbit polyclonal antibody to NDUFA8Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NDUFA8. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol:NDUFA8Alternative Names:NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; Complex I-19kD; CI-19kD; Complex I-PGIV; CI-PGIV; NADH-ubiquinone oxidoreductase 19 kDa subunit
Entrez Gene (Human):
4702;
Entrez Gene (Mouse):
68375;
SwissProt (Human):
P51970;
SwissProt (Mouse):
Q9DCJ5;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NDUFA8 expression in HEK293T (A), Hela (B), A2780 (C), HepG2 (D), mouse muscle (E), mouse kidney (F), rat kidney (G) whole cell lysates. (Predicted band size: 20 kD; Observed band size: 20 kD)
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Immunohistochemical analysis of NDUFA8 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.