Description:Rabbit polyclonal antibody to MT-ND1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MT-ND1. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol:MT-ND1Alternative Names:MTND1; NADH1; ND1; NADH-ubiquinone oxidoreductase chain 1; NADH dehydrogenase subunit 1
Entrez Gene (Human):
4535;
SwissProt (Human):
P03886;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MT-ND1 expression in HCT116 (A), Hela (B), BV2 (C), H9C2 (D) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 36 kD)
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Immunohistochemical analysis of MT-ND1 staining in human placenta formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Interactions between PBEF and oxidative stress proteins--a potential new mechanism underlying PBEF in the pathogenesis of acute lung injury
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