Description：Rabbit polyclonal antibody to ATP6Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATP6. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：MT-ATP6Alternative Names：ATP6; ATPASE6; MTATP6; ATP synthase subunit a; F-ATPase protein 6 Entrez Gene (Human)： 4508; SwissProt (Human)： P00846; SwissProt (Rat)： P05504; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ATP6 expression in HEK293T (A), HCT116 (B), A375 (C), PC12 (D), CT26 (E) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD)
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  Immunofluorescent analysis of ATP6 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).