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CPA1745
  • Western blot analysis of FOXO4 expression in HeLa (A), MM142 (B), HuT78 (C), NIH3T3 (D) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 65 kD)
  • Immunohistochemical analysis of FOXO4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-FOXO4 Antibody
Cat No:CPA1745
Source:Rabbit
Reactivity:H, M, R, Mk, P
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to FOXO4
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FOXO4. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol:FOXO4
Alternative Names:AFX; AFX1; MLLT7; Forkhead box protein O4; Fork head domain transcription factor AFX1
Entrez Gene (Human): 4303;
Entrez Gene (Mouse): 54601;
SwissProt (Human): P98177;
SwissProt (Mouse): Q9WVH3;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of FOXO4 expression in HeLa (A), MM142 (B), HuT78 (C), NIH3T3 (D) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 65 kD)
  • Immunohistochemical analysis of FOXO4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Transforming growth factor-beta regulates basal transcriptional regulatory machinery to control cell proliferation and differentiation in cranial neural crest-derived osteoprogenitor cells
Journal J Biol Chem
IF 4.8
Application WB
Reactivity Mouse
PMID 19959467
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