Description:Rabbit polyclonal antibody to MCL1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MCL1. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:MCL1Alternative Names:BCL2L3; Induced myeloid leukemia cell differentiation protein Mcl-1; Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT
Entrez Gene (Human):
4170;
SwissProt (Human):
Q07820;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MCL1 expression in Ramos (A), JAR (B), K562 (C), Jurkat (D), Raji (E), HeLa (F) whole cell lysates. (Predicted band size: 37 kD; Observed band size: 40 kD)
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Immunohistochemical analysis of MCL1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Preapoptotic cell stress response of primary hepatocytes
Regulation of serum response factor activity and smooth muscle cell apoptosis by chromodomain helicase DNA-binding protein 8