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CPA1726
  • Western blot analysis of MCL1 expression in Ramos (A), JAR (B), K562 (C), Jurkat (D), Raji (E), HeLa (F) whole cell lysates. (Predicted band size: 37 kD; Observed band size: 40 kD)
  • Immunohistochemical analysis of MCL1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-MCL1 Antibody
Cat No:CPA1726
Source:Rabbit
Reactivity:H, D, P
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to MCL1
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MCL1. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol:MCL1
Alternative Names:BCL2L3; Induced myeloid leukemia cell differentiation protein Mcl-1; Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT
Entrez Gene (Human): 4170;
SwissProt (Human): Q07820;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of MCL1 expression in Ramos (A), JAR (B), K562 (C), Jurkat (D), Raji (E), HeLa (F) whole cell lysates. (Predicted band size: 37 kD; Observed band size: 40 kD)
  • Immunohistochemical analysis of MCL1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Preapoptotic cell stress response of primary hepatocytes
Journal Hepatology
IF 13.5
Application WB, ICC/IF
Reactivity Rat
PMID 20513000
Regulation of serum response factor activity and smooth muscle cell apoptosis by chromodomain helicase DNA-binding protein 8
Journal AM J PHYSIOL-CELL PH
IF 5.5
Application WB
Reactivity Mouse
PMID 20739623
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