Description:Rabbit polyclonal antibody to LTBRImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human LTBR. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:LTBRAlternative Names:D12S370; TNFCR; TNFR3; TNFRSF3; Tumor necrosis factor receptor superfamily member 3; Lymphotoxin-beta receptor; Tumor necrosis factor C receptor; Tumor necrosis factor receptor 2-related protein; Tumor necrosis factor receptor type III; TNF-RIII; TNFR-III
Entrez Gene (Human):
4055;
Entrez Gene (Mouse):
17000;
SwissProt (Human):
P36941;
SwissProt (Mouse):
P50284;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of LTBR expression in HEK293T (A), Hela (B), DLD (C), mouse brain (D), mouse testis (E), rat brain (F), rat testis (G) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 55 kD)
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Immunofluorescent analysis of LTBR staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.