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CPA1689
  • Western blot analysis of HSL expression in Panc1 (A), SKOVCAR3 (B), C6 (C), 3T3L1 (D) whole cell lysates. (Predicted band size: 116 kD; Observed band size: 120; 88 kD)
  • Immunohistochemical analysis of HSL staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-HSL Antibody
Cat No:CPA1689
Source:Rabbit
Reactivity:H, M, R, Mk
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to HSL
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSL. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)
Gene Symbol:LIPE
Alternative Names:Hormone-sensitive lipase; HSL
Entrez Gene (Human): 3991;
SwissProt (Human): Q05469;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of HSL expression in Panc1 (A), SKOVCAR3 (B), C6 (C), 3T3L1 (D) whole cell lysates. (Predicted band size: 116 kD; Observed band size: 120; 88 kD)
  • Immunohistochemical analysis of HSL staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Insulin-containing lipogenic stimuli suppress mast cell degranulation potential and up-regulate lipid body biogenesis and eicosanoid secretion in a PPAR?-independent manner
Journal J Leukoc Biol
IF 5.5
Application
Reactivity
PMID 22706316
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