Welcome to Cohesion Biosciences
Home> Products > Primary Antibody
CPA1673
  • Western blot analysis of LCK (Phospho-Y393) expression in Myla2059 (A), HuT78 (B) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 56 kD)
  • Immunofluorescent analysis of LCK (Phospho-Y393) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
  • Direct ELISA antibody dose-response curve using Anti-LCK (Phospho-Y393) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
Product Name:Anti-LCK (Phospho-Y393) Antibody
Cat No:CPA1673
Source:Rabbit
Reactivity:H, M, R, B, C, P, S
Applications:WB
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
420
100 μl
260
30 μl
130
50 μl
170
Add to cart Ship in 3 days My orders
Description:Rabbit polyclonal antibody to LCK (Phospho-Y393)
Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y393 of human LCK protein. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000)
Gene Symbol:LCK
Alternative Names:Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK
Entrez Gene (Human): 3932;
Entrez Gene (Mouse): 16818;
Entrez Gene (Rat): 313050;
SwissProt (Human): P06239;
SwissProt (Mouse): P06240;
SwissProt (Rat): Q01621;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of LCK (Phospho-Y393) expression in Myla2059 (A), HuT78 (B) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 56 kD)
  • Immunofluorescent analysis of LCK (Phospho-Y393) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
  • Direct ELISA antibody dose-response curve using Anti-LCK (Phospho-Y393) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
COHESION BIOSCIENCES LIMITED
Copyright © 2022 Cohesion Biosciences. All Rights Reserved