Description：Rabbit polyclonal antibody to LCK (Phospho-Y393)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y393 of human LCK protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000)Gene Symbol：LCKAlternative Names：Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK Entrez Gene (Human)： 3932; Entrez Gene (Mouse)： 16818; Entrez Gene (Rat)： 313050; SwissProt (Human)： P06239; SwissProt (Mouse)： P06240; SwissProt (Rat)： Q01621; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of LCK (Phospho-Y393) expression in Myla2059 (A), HuT78 (B) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 56 kD)
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  Immunofluorescent analysis of LCK (Phospho-Y393) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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  Direct ELISA antibody dose-response curve using Anti-LCK (Phospho-Y393) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.