Description:Rabbit polyclonal antibody to Laminin alpha 5Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Laminin alpha 5. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)Gene Symbol:LAMA5Alternative Names:KIAA0533; KIAA1907; Laminin subunit alpha-5; Laminin-10 subunit alpha; Laminin-11 subunit alpha; Laminin-15 subunit alpha
Entrez Gene (Human):
3911;
Entrez Gene (Mouse):
16776;
SwissProt (Human):
O15230;
SwissProt (Mouse):
Q61001;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Laminin alpha 5 expression in JAR (A), A431 (B), RAW264.7 (C) whole cell lysates. (Predicted band size: 399 kD; Observed band size: 400 kD)
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Immunohistochemical analysis of Laminin alpha 5 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.