Description：Rabbit polyclonal antibody to Cytokeratin 18 (Phospho-S52)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S52 of human Cytokeratin 18 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200)Gene Symbol：KRT18Alternative Names：CYK18; Keratin, type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18 Entrez Gene (Human)： 3875; Entrez Gene (Mouse)： 16668; Entrez Gene (Rat)： 294853; SwissProt (Human)： P05783; SwissProt (Mouse)： P05784; SwissProt (Rat)： Q5BJY9; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Cytokeratin 18 (Phospho-S52) expression in HEK293T (A), Hela (B), DLD (C) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 46 kD)
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  Immunohistochemical analysis of Cytokeratin 18 (Phospho-S52) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of Cytokeratin 18 (Phospho-S52) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.