Description:Rabbit polyclonal antibody to CD51 HCImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD51 HC. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:ITGAVAlternative Names:MSK8; VNRA; Integrin alpha-V; Vitronectin receptor subunit alpha; CD51
Entrez Gene (Human):
3685;
SwissProt (Human):
P06756;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD51 HC expression in A549 (A), Hela (B), C6 (C), MEF (D) whole cell lysates. (Predicted band size: 116 kD; Observed band size: 125; 95 kD)
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Immunohistochemical analysis of CD51 HC staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Western blot analysis of CD51 HC expression in wild type (WT) and knockdown (KD) HT1080 cell lysates.