Description：Rabbit polyclonal antibody to IRS1 (Phospho-S307)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S307 of human IRS1 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol：IRS1Alternative Names：Insulin receptor substrate 1; IRS-1 Entrez Gene (Human)： 3667; Entrez Gene (Mouse)： 16367; Entrez Gene (Rat)： 25467; SwissProt (Human)： P35568; SwissProt (Mouse)： P35569; SwissProt (Rat)： P35570; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of IRS1 (Phospho-S307) expression in HEK293T insulin-treated (A) whole cell lysates. (Predicted band size: 131 kD; Observed band size: 180 kD)
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  Immunohistochemical analysis of IRS1 (Phospho-S307) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.