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CPA1570
  • Western blot analysis of CD54 expression in A549 (A), HepG2 (B), AML12 (C) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 92; 100 kD)
  • Immunohistochemical analysis of CD54 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-CD54 Antibody
Cat No:CPA1570
Source:Rabbit
Reactivity:H, M
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to CD54
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD54. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)
Gene Symbol:ICAM1
Alternative Names:Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD54
Entrez Gene (Human): 3383;
Entrez Gene (Mouse): 15894;
SwissProt (Human): P05362;
SwissProt (Mouse): P13597;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of CD54 expression in A549 (A), HepG2 (B), AML12 (C) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 92; 100 kD)
  • Immunohistochemical analysis of CD54 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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