Description:Rabbit polyclonal antibody to HSD11B1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human HSD11B1. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol:HSD11B1Alternative Names:HSD11; HSD11L; Corticosteroid 11-beta-dehydrogenase isozyme 1; 11-beta-hydroxysteroid dehydrogenase 1; 11-DH; 11-beta-HSD1
Entrez Gene (Human):
3290;
Entrez Gene (Rat):
25116;
SwissProt (Human):
P28845;
SwissProt (Rat):
P16232;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HSD11B1 expression in human liver (A) whole cell lysates. (Predicted band size: 32 kD; Observed band size: 32 kD)
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Immunohistochemical analysis of HSD11B1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.