Description:Rabbit polyclonal antibody to GSTM2Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GSTM2. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:GSTM2Alternative Names:GST4; Glutathione S-transferase Mu 2; GST class-mu 2; GSTM2-2
Entrez Gene (Human):
2946;
Entrez Gene (Mouse):
14863;
Entrez Gene (Rat):
24424;
SwissProt (Human):
P28161;
SwissProt (Mouse):
P15626;
SwissProt (Rat):
P08010;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GSTM2 expression in HepG2 (A), HeLa (B), mouse liver (C), rat liver (D) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 25 kD)
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Immunofluorescent analysis of GSTM2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).