Description:Rabbit polyclonal antibody to GSK3 alpha/betaImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GSK3 alpha/beta. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:GSK3A; GSK3BAlternative Names:GSK3A; Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
Entrez Gene (Human):
2931;
Entrez Gene (Mouse):
606496;
56637;
Entrez Gene (Rat):
50686;
84027;
SwissProt (Human):
P49840;
P49841;
SwissProt (Mouse):
Q2NL51;
Q9WV60;
SwissProt (Rat):
P18265;
P18266;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GSK3 alpha/beta expression in 293T (A), A549 (B), Hela (C), mouse brain (D), rat brain (E) whole cell lysates. (Predicted band size: 50; 46 kD; Observed band size: 51; 46 kD)
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Immunohistochemical analysis of GSK3 alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.