Description:Rabbit polyclonal antibody to GPR17Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR17. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:GPR17Alternative Names:Uracil nucleotide/cysteinyl leukotriene receptor; UDP/CysLT receptor; G-protein coupled receptor 17; P2Y-like receptor; R12
Entrez Gene (Human):
2840;
Entrez Gene (Mouse):
574402;
Entrez Gene (Rat):
767613;
SwissProt (Human):
Q13304;
SwissProt (Mouse):
Q6NS65;
SwissProt (Rat):
Q09QM4;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GPR17 expression in HEK293T (A), Jurkat (B), NIH3T3 (C), rat kidney (D) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 40 kD)
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Immunofluorescent analysis of GPR17 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.