Description:Rabbit polyclonal antibody to Connexin 32Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Connexin 32. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:GJB1Alternative Names:CX32; Gap junction beta-1 protein; Connexin-32; Cx32; GAP junction 28 kDa liver protein
Entrez Gene (Human):
2705;
Entrez Gene (Mouse):
14618;
Entrez Gene (Rat):
29584;
SwissProt (Human):
P08034;
SwissProt (Mouse):
P28230;
SwissProt (Rat):
P08033;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Connexin 32 expression in LOVO (A), mouse brain (B), mouse lung (C), mouse kidney (D), rat kidney (E) whole cell lysates. (Predicted band size: 32 kD; Observed band size: 28 kD)
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Immunohistochemical analysis of Connexin 32 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.