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CPA1434
  • Western blot analysis of Filamin A expression in HEK293T (A), SP2/0 (B), rat heart (C) whole cell lysates. (Predicted band size: 280 kD; Observed band size: 300 kD)
  • Immunohistochemical analysis of Filamin A staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-Filamin A Antibody
Cat No:CPA1434
Source:Rabbit
Reactivity:H, M, R, Rb
Applications:WB, IH, IP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to Filamin A
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Filamin A. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
Gene Symbol:FLNA
Alternative Names:FLN; FLN1; Filamin-A; FLN-A; Actin-binding protein 280; ABP-280; Alpha-filamin; Endothelial actin-binding protein; Filamin-1; Non-muscle filamin
Entrez Gene (Human): 2316;
Entrez Gene (Mouse): 192176;
SwissProt (Human): P21333;
SwissProt (Mouse): Q8BTM8;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Filamin A expression in HEK293T (A), SP2/0 (B), rat heart (C) whole cell lysates. (Predicted band size: 280 kD; Observed band size: 300 kD)
  • Immunohistochemical analysis of Filamin A staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Filamin A is a phosphorylation target of membrane but not cytosolic adenylyl cyclase activity
Journal AM J PHYSIOL-LUNG C
IF 4.9
Application WB, ICC/IF
Reactivity Rat
PMID 21478251
Filamin a protein interacts with human immunodeficiency virus type 1 gag protein and contributes to productive particle assembly
Journal J Biol Chem
IF 4.8
Application Flow Cyt
Reactivity Human
PMID 21705339
Enhancing the effectiveness of androgen deprivation in prostate cancer by inducing Filamin A nuclear localization
Journal Endocr Relat Cancer
IF 3.9
Application ICC/IF
Reactivity Human
PMID 22993077
RSK2 Protein Suppresses Integrin Activation and Fibronectin Matrix Assembly and Promotes Cell Migration
Journal J Biol Chem
IF 4.8
Application WB
Reactivity
PMID 23118220
Filamin-interacting proteins, Cfm1 and Cfm2, are essential for the formation of cartilaginous skeletal elements
Journal Hum Mol Genet
IF 3.5
Application
Reactivity Mouse
PMID 24436304
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