Description:Rabbit polyclonal antibody to FABP1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FABP1. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:FABP1Alternative Names:FABPL; Fatty acid-binding protein, liver; Fatty acid-binding protein 1; Liver-type fatty acid-binding protein; L-FABP
Entrez Gene (Human):
2168;
Entrez Gene (Rat):
24360;
SwissProt (Human):
P07148;
SwissProt (Rat):
P02692;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of FABP1 expression in HeLa (A), Jurkat (B), rat liver (C) whole cell lysates. (Predicted band size: 14 kD; Observed band size: 14 kD)
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Immunofluorescent analysis of FABP1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).