Description：Rabbit polyclonal antibody to EZH1Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EZH1. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：EZH1Alternative Names：KIAA0388; Histone-lysine N-methyltransferase EZH1; ENX-2; Enhancer of zeste homolog 1 Entrez Gene (Human)： 2145; Entrez Gene (Mouse)： 14055; SwissProt (Human)： Q92800; SwissProt (Mouse)： P70351; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EZH1 expression in HepG2 (A), HeLa (B), MOLT4 (C), mouse brain (D) whole cell lysates. (Predicted band size: 68; 76; 80; 85; 86 kD; Observed band size: 85 kD)
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  Immunofluorescent analysis of EZH1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.