Description:Rabbit polyclonal antibody to EIF2S1 (Phospho-S51)Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S51 of human EIF2S1 protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol:EIF2S1Alternative Names:EIF2A; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha
Entrez Gene (Human):
1965;
Entrez Gene (Mouse):
13665;
Entrez Gene (Rat):
54318;
SwissProt (Human):
P05198;
SwissProt (Mouse):
Q6ZWX6;
SwissProt (Rat):
P68101;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EIF2S1 (Phospho-S51) expression in A549 (A), mouse kidney (B), mouse lung (C) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 38 kD)
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Immunohistochemical analysis of EIF2S1 (Phospho-S51) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.