Description：Rabbit polyclonal antibody to EGFRImmunogen：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EGFR. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol：EGFRAlternative Names：ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 Entrez Gene (Human)： 1956; Entrez Gene (Mouse)： 13649; SwissProt (Human)： P00533; SwissProt (Mouse)： Q01279; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of EGFR expression in H1792 (A), A549 (B), A2780 (C) whole cell lysates. (Predicted band size: 134 kD; Observed band size: 175 kD)
• 
  Immunohistochemical analysis of EGFR staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.