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CPA1347
  • Western blot analysis of Dynamin 1 (Phospho-S774) expression in mouse brain (A), mouse kidney (B), rat brain (C) whole cell lysates. (Predicted band size: 97 kD; Observed band size: 100; 120 kD)
  • Immunohistochemical analysis of Dynamin 1 (Phospho-S774) staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-Dynamin 1 (Phospho-S774) Antibody
Cat No:CPA1347
Source:Rabbit
Reactivity:H, M, R, D
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
420
100 μl
260
30 μl
130
50 μl
170
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Description:Rabbit polyclonal antibody to Dynamin 1 (Phospho-S774)
Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S774 of human Dynamin 1 protein. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)
Gene Symbol:DNM1
Alternative Names:DNM; Dynamin-1
Entrez Gene (Human): 1759;
Entrez Gene (Mouse): 13429;
Entrez Gene (Rat): 140694;
SwissProt (Human): Q05193;
SwissProt (Mouse): P39053;
SwissProt (Rat): P21575;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Dynamin 1 (Phospho-S774) expression in mouse brain (A), mouse kidney (B), rat brain (C) whole cell lysates. (Predicted band size: 97 kD; Observed band size: 100; 120 kD)
  • Immunohistochemical analysis of Dynamin 1 (Phospho-S774) staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Transmembrane/cytoplasmic, rather than catalytic, domains of Mmp14 signal to MAPK activation and mammary branching morphogenesis via binding to integrin ß1
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