Description:Rabbit polyclonal antibody to Dynamin 1 (Phospho-S774)Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S774 of human Dynamin 1 protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol:DNM1Alternative Names:DNM; Dynamin-1
Entrez Gene (Human):
1759;
Entrez Gene (Mouse):
13429;
Entrez Gene (Rat):
140694;
SwissProt (Human):
Q05193;
SwissProt (Mouse):
P39053;
SwissProt (Rat):
P21575;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Dynamin 1 (Phospho-S774) expression in mouse brain (A), mouse kidney (B), rat brain (C) whole cell lysates. (Predicted band size: 97 kD; Observed band size: 100; 120 kD)
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Immunohistochemical analysis of Dynamin 1 (Phospho-S774) staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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