Description:Rabbit polyclonal antibody to Cytochrome P450 26A1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome P450 26A1. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)Gene Symbol:CYP26A1Alternative Names:CYP26; P450RAI1; Cytochrome P450 26A1; Cytochrome P450 retinoic acid-inactivating 1; Cytochrome P450RAI; hP450RAI; Retinoic acid 4-hydroxylase; Retinoic acid-metabolizing cytochrome
Entrez Gene (Human):
1592;
Entrez Gene (Mouse):
13082;
SwissProt (Human):
O43174;
SwissProt (Mouse):
O55127;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cytochrome P450 26A1 expression in HepG2 (A), HEK293T (B) whole cell lysates. (Predicted band size: 48; 56 kD; Observed band size: 55 kD)
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Immunofluorescent analysis of Cytochrome P450 26A1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).