Description：Rabbit polyclonal antibody to Cytochrome P450 8B1Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome P450 8B1. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol：CYP8B1Alternative Names：CYP12; 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; CYPVIIIB1; Cytochrome P450 8B1; Sterol 12-alpha-hydroxylase Entrez Gene (Human)： 1582; Entrez Gene (Mouse)： 13124; SwissProt (Human)： Q9UNU6; SwissProt (Mouse)： O88962; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Cytochrome P450 8B1 expression in mouse liver (A), mouse kidney (B), rat liver (C), rat kidney (D) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 58 kD)
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  Immunohistochemical analysis of Cytochrome P450 8B1 staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.