Description:Rabbit polyclonal antibody to Cardiotrophin-1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Cardiotrophin-1. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:CTF1Alternative Names:Cardiotrophin-1; CT-1
Entrez Gene (Human):
1489;
Entrez Gene (Mouse):
13019;
Entrez Gene (Rat):
29201;
SwissProt (Human):
Q16619;
SwissProt (Mouse):
Q60753;
SwissProt (Rat):
Q63086;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Cardiotrophin-1 expression in Hela (A), mouse lung (B), rat lung (C), rat testis (D) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 25 kD)
-
Immunohistochemical analysis of Cardiotrophin-1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.