Description:Rabbit polyclonal antibody to NeurocanImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Neurocan. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol:NCANAlternative Names:CSPG3; NEUR; Neurocan core protein; Chondroitin sulfate proteoglycan 3
Entrez Gene (Human):
1463;
Entrez Gene (Mouse):
13004;
SwissProt (Human):
O14594;
SwissProt (Mouse):
P55066;
SwissProt (Rat):
P55067;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Neurocan expression in CT26 (A), HCT116 (B), MCF7 (C), HepG2 (D) whole cell lysates. (Predicted band size: 143 kD; Observed band size: 143 kD)
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Immunohistochemical analysis of Neurocan staining in human tonsillitis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.