Welcome to Cohesion Biosciences
Home> Products > Primary Antibody
CPA1283
  • Western blot analysis of CK1 gamma 2 expression in HeLa (A), Raw264.7 (B), PC12 (C), rat heart (D) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD)
  • Immunofluorescent analysis of CK1 gamma 2 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Product Name:Anti-CK1 gamma 2 Antibody
Cat No:CPA1283
Source:Rabbit
Reactivity:H, M, R, B
Applications:WB, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
Add to cart Ship in 3 days My orders
Description:Rabbit polyclonal antibody to CK1 gamma 2
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CK1 gamma 2. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Gene Symbol:CSNK1G2
Alternative Names:CK1G2; Casein kinase I isoform gamma-2; CKI-gamma 2
Entrez Gene (Human): 1455;
Entrez Gene (Mouse): 103236;
Entrez Gene (Rat): 65278;
SwissProt (Human): P78368;
SwissProt (Mouse): Q8BVP5;
SwissProt (Rat): Q62762;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of CK1 gamma 2 expression in HeLa (A), Raw264.7 (B), PC12 (C), rat heart (D) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD)
  • Immunofluorescent analysis of CK1 gamma 2 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
A p120-catenin-CK1epsilon complex regulates Wnt signaling
Journal J Cell Sci
IF 4
Application
Reactivity
PMID 20940130
Coordinated Action of CK1 Isoforms in Canonical Wnt Signaling
Journal Mol Cell Biol
IF 5.3
Application
Reactivity
PMID 21606194
COHESION BIOSCIENCES LIMITED
Copyright © 2022 Cohesion Biosciences. All Rights Reserved