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CPA1272
  • Western blot analysis of ATF2 expression in BV2 (A), C6 (B), Hela (C), HEK293T (D), MCF7 (E) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 54 kD)
  • Immunohistochemical analysis of ATF2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-ATF2 Antibody
Cat No:CPA1272
Source:Rabbit
Reactivity:H, M, R, C
Applications:WB, IH, IP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to ATF2
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ATF2. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
Gene Symbol:ATF2
Alternative Names:CREB2; CREBP1; Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; HB16; Histone acetyltransferase ATF2; cAMP response element-binding protein CRE-BP1
Entrez Gene (Human): 1386;
Entrez Gene (Mouse): 102641666; 11909;
Entrez Gene (Rat): 81647;
SwissProt (Human): P15336;
SwissProt (Mouse): P16951;
SwissProt (Rat): Q00969;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of ATF2 expression in BV2 (A), C6 (B), Hela (C), HEK293T (D), MCF7 (E) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 54 kD)
  • Immunohistochemical analysis of ATF2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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