Description：Rabbit polyclonal antibody to CREB (Phospho-S133)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S133 of human CREB protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)Gene Symbol：CREB1Alternative Names：Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1 Entrez Gene (Human)： 1385; Entrez Gene (Mouse)： 12912; Entrez Gene (Rat)： 81646; SwissProt (Human)： P16220; SwissProt (Mouse)： Q01147; SwissProt (Rat)： P15337; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CREB (Phospho-S133) expression in C6 (A), NIH3T3 (B), HEK293T (C), H1792 (D) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 43 kD)
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  Immunohistochemical analysis of CREB (Phospho-S133) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Direct ELISA antibody dose-response curve using Anti-CREB (Phospho-S133) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.