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CPA1223
  • Western blot analysis of CHK1 (Phospho-S286) expression in HEK293T (A), Jurkat (B) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 60 kD)
  • Immunofluorescent analysis of CHK1 (Phospho-S286) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Product Name:Anti-CHK1 (Phospho-S286) Antibody
Cat No:CPA1223
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
420
100 μl
260
30 μl
130
50 μl
170
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Description:Rabbit polyclonal antibody to CHK1 (Phospho-S286)
Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S286 of human CHK1 protein. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
Gene Symbol:CHEK1
Alternative Names:CHK1; Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1
Entrez Gene (Human): 1111;
Entrez Gene (Mouse): 12649;
Entrez Gene (Rat): 140583;
SwissProt (Human): O14757;
SwissProt (Mouse): O35280;
SwissProt (Rat): Q91ZN7;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of CHK1 (Phospho-S286) expression in HEK293T (A), Jurkat (B) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 60 kD)
  • Immunofluorescent analysis of CHK1 (Phospho-S286) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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