Description：Rabbit polyclonal antibody to CHK1 (Phospho-S286)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S286 of human CHK1 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：CHEK1Alternative Names：CHK1; Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1 Entrez Gene (Human)： 1111; Entrez Gene (Mouse)： 12649; Entrez Gene (Rat)： 140583; SwissProt (Human)： O14757; SwissProt (Mouse)： O35280; SwissProt (Rat)： Q91ZN7; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CHK1 (Phospho-S286) expression in HEK293T (A), Jurkat (B) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 60 kD)
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  Immunofluorescent analysis of CHK1 (Phospho-S286) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.