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CPA1194
  • Western blot analysis of CDC25A (Phospho-S75) expression in HEK293T UV-treated (A), Jurkat UV-treated (B), NIH3T3 (C) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 70 kD)
  • Immunohistochemical analysis of CDC25A (Phospho-S75) staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-CDC25A (Phospho-S75) Antibody
Cat No:CPA1194
Source:Rabbit
Reactivity:H
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
420
100 μl
260
30 μl
130
50 μl
170
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Description:Rabbit polyclonal antibody to CDC25A (Phospho-S75)
Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S75 of human CDC25A protein. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol:CDC25A
Alternative Names:M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A
Entrez Gene (Human): 993;
SwissProt (Human): P30304;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of CDC25A (Phospho-S75) expression in HEK293T UV-treated (A), Jurkat UV-treated (B), NIH3T3 (C) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 70 kD)
  • Immunohistochemical analysis of CDC25A (Phospho-S75) staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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