Description:Rabbit polyclonal antibody to Cyclin B1 (Phospho-S126)Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S126 of human Cyclin B1 protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol:CCNB1Alternative Names:CCNB; G2/mitotic-specific cyclin-B1
Entrez Gene (Human):
891;
Entrez Gene (Mouse):
268697;
SwissProt (Human):
P14635;
SwissProt (Mouse):
P24860;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cyclin B1 (Phospho-S126) expression in K562 (A) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 60 kD)
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Immunohistochemical analysis of Cyclin B1 (Phospho-S126) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Role of Filia, a maternal effect gene, in maintaining euploidy during cleavage-stage mouse embryogenesis
Novel positive feedback loop between Cdk1 and Chk1 in the nucleus during G2/M transition
Greatwall is essential to prevent mitotic collapse after nuclear envelope breakdown in mammals