Description：Rabbit polyclonal antibody to Calcium Sensing Receptor (Phospho-T888)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T888 of human Calcium Sensing Receptor protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：CASRAlternative Names：GPRC2A; PCAR1; Extracellular calcium-sensing receptor; CaSR; Parathyroid cell calcium-sensing receptor 1; PCaR1 Entrez Gene (Human)： 846; Entrez Gene (Mouse)： 12374; Entrez Gene (Rat)： 24247; SwissProt (Human)： P41180; SwissProt (Mouse)： Q9QY96; SwissProt (Rat)： P48442; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Calcium Sensing Receptor (Phospho-T888) expression in MCF7 (A), MDCK (B), U2OS (C), SP2/0 (D), PC12 (E), rat brain (F) whole cell lysates. (Predicted band size: 120 kD; Observed band size: 140 kD)
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  Immunofluorescent analysis of Calcium Sensing Receptor (Phospho-T888) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.