Description：Rabbit polyclonal antibody to Caspase 8 (Phospho-Y380)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y380 of human Caspase 8 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：CASP8Alternative Names：MCH5; Caspase-8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH Entrez Gene (Human)： 841; SwissProt (Human)： Q14790; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Caspase 8 (Phospho-Y380) expression in HepG2 (A), Jurkat (B), mouse spleen (C) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 57; 18 kD)
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  Immunofluorescent analysis of Caspase 8 (Phospho-Y380) staining in B12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).