Description:Rabbit polyclonal antibody to Caspase 7 p11Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Caspase 7. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol:CASP7Alternative Names:MCH3; Caspase-7; CASP-7; Apoptotic protease Mch-3; CMH-1; ICE-like apoptotic protease 3; ICE-LAP3
Entrez Gene (Human):
840;
Entrez Gene (Mouse):
12369;
SwissProt (Human):
P55210;
SwissProt (Mouse):
P97864;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Caspase 7 p11 expression in HEK293T (A), mouse spleen (B), rat spleen (C) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 11 kD)
-
Immunofluorescent analysis of Caspase 7 p11 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).