Description：Rabbit polyclonal antibody to Carbonic Anhydrase 5AImmunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Carbonic Anhydrase 5A. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：CA5AAlternative Names：CA5; Carbonic anhydrase 5A mitochondrial; Carbonate dehydratase VA; Carbonic anhydrase VA; CA-VA Entrez Gene (Human)： 763; Entrez Gene (Mouse)： 12352; Entrez Gene (Rat)： 54233; SwissProt (Human)： P35218; SwissProt (Mouse)： P23589; SwissProt (Rat)： P43165; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Carbonic Anhydrase 5A expression in HEK293T (A), mouse liver (B), rat liver (C), rat spleen (D) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 30 kD)
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  Immunofluorescent analysis of Carbonic Anhydrase 5A staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).