Description:Rabbit polyclonal antibody to CD66a/eImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CD66a/e. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol:CEACAM1; CEACAM5Alternative Names:CEACAM1; BGP; BGP1; Carcinoembryonic antigen-related cell adhesion molecule 1; Biliary glycoprotein 1; BGP-1; CD66a; CEACAM5; CEA; Carcinoembryonic antigen-related cell adhesion molecule 5; Carcinoembryonic antigen; CEA; Meconium antigen 100; CD66e
Entrez Gene (Human):
634;
1048;
SwissProt (Human):
P13688;
P06731;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD66a/e expression in HUT78 (A), HCT116 (B) whole cell lysates. (Predicted band size: 57; 76 kD; Observed band size: 180 kD)
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Immunohistochemical analysis of CD66a/e staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.