Description：Rabbit polyclonal antibody to ATF4Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATF4. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol：ATF4Alternative Names：CREB2; TXREB; Cyclic AMP-dependent transcription factor ATF-4; cAMP-dependent transcription factor ATF-4; Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67 Entrez Gene (Human)： 468; Entrez Gene (Mouse)： 11911; Entrez Gene (Rat)： 79255; SwissProt (Human)： P18848; SwissProt (Mouse)： Q06507; SwissProt (Rat)： Q9ES19; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ATF4 expression in SGC7901 (A), C6 (B), EC7901 (C) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 55 kD)
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  Immunohistochemical analysis of ATF4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.