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Western blot analysis of ARF6 expression in HEK293T (A), Hela (B), A2780 (C), mouse liver (D), mouse spleen (E), rat liver (F), rat spleen (G) whole cell lysates. (Predicted band size: 20 kD; Observed band size: 20 kD)
Immunohistochemical analysis of ARF6 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ARF6 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Name:	Anti-ARF6 Antibody
Cat No:	CPA1073
Source:	Rabbit
Reactivity:	H, M, R, C, P, Z
Applications:	WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

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200 μl

350

100 μl

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30 μl

110

50 μl

150

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Datasheet

MSDS

Description：Rabbit polyclonal antibody to ARF6

Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ARF6. The exact sequence is proprietary.

Purification：The antibody was purified by immunogen affinity chromatography.

Clonality：Polyclonal

Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200)

Gene Symbol：ARF6

Alternative Names：ADP-ribosylation factor 6

Entrez Gene (Human)： 382;

Entrez Gene (Mouse)： 11845;

Entrez Gene (Rat)： 79121;

SwissProt (Human)： P62330;

SwissProt (Mouse)： P62331;

SwissProt (Rat)： P62332;

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of ARF6 expression in HEK293T (A), Hela (B), A2780 (C), mouse liver (D), mouse spleen (E), rat liver (F), rat spleen (G) whole cell lysates. (Predicted band size: 20 kD; Observed band size: 20 kD)
Immunohistochemical analysis of ARF6 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ARF6 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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