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CPA1056
  • Western blot analysis of APC (Phospho-S2054) expression in HeLa (A), Caco2 (B), SW480 (C), MCF7 (D), SP2/0 (E), PC12 (F) whole cell lysates. (Predicted band size: 311 kD; Observed band size: 310 kD)
  • Immunohistochemical analysis of APC (Phospho-S2054) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-APC (Phospho-S2054) Antibody
Cat No:CPA1056
Source:Rabbit
Reactivity:H, M, R, Mk, P
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
420
100 μl
260
30 μl
130
50 μl
170
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Description:Rabbit polyclonal antibody to APC (Phospho-S2054)
Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S2054 of human APC protein. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)
Gene Symbol:APC
Alternative Names:DP2.5; Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5
Entrez Gene (Human): 324;
Entrez Gene (Rat): 24205;
SwissProt (Human): P25054;
SwissProt (Mouse): Q61315;
SwissProt (Rat): P70478;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of APC (Phospho-S2054) expression in HeLa (A), Caco2 (B), SW480 (C), MCF7 (D), SP2/0 (E), PC12 (F) whole cell lysates. (Predicted band size: 311 kD; Observed band size: 310 kD)
  • Immunohistochemical analysis of APC (Phospho-S2054) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Mammalian diaphanous-related formin 1 regulates GSK3ß-dependent microtubule dynamics required for T cell migratory polarization
Journal PLoS One
IF 3.7
Application
Reactivity Mouse
PMID 24260404
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